Genes and strains
Gene synthesis based on codon preference of each host, molecular techniques using restriction enzymes and gene transfer are an integral part of recombinant enzyme production. These steps, apart from their costs, are demanding process. To facilitate these stages and also reduce the costs and time of research, Dana Co. provides and offers some easy-to-use products and services at three levels; proteins expression in lab and large scale, synthesized and prepared nucleotide sequences and supplying transformed hosts.
The below hosts are bacterial and yeast strains used to produce recombinant proteins. Depending on the type of strain, their production capacity is laboratory, semi-industrial and in some cases industrial.
| Description | Name | Row |
| Expression Host | Hansenulla polymorpha KL8 | 1 |
| Expression Host | Pichia pastoris X33 (Humanized glycosylation ) | 2 |
| Expression Host | Pichia pastoris x33 | 3 |
| Expression Host | Pichia pastoris | 4 |
| Expression Host | Arxula adeninivorans | 5 |
| Expression Host | Ecoli BL21 | 6 |
| Expression Host | Ecoli DH5α | 7 |
The nucleotide sequence of each of the genes listed in the table was synthesized based on the codon preference of the respective host.
These sequences were synthesized based on the codon preference of the mentioned host.
These services can be provided in these levels
- Purified gens in expression and synthetic vectors.
- Transformed host expression in solid medium.
- Protein or enzyme products.
Description | Row |
Target Protein: DNA Polymeras KOD Source: Thermococcus kodakaraensis Host: E.coli BL21 Vector: pET 21 Capacity: Semi-industrial | 1 |
Target Protein: Cellobiohydrolases I (CBH I) :Source Host: Pichia pastoris Vector: pPink α- HC Capacity: Laboratory | 2 |
Target Protein: endoglucanase II (EG II) Source: Trichoderma reesei Host: Pichia pastoris Vector: pPink α- HC Capacity: Industrial | 3 |
Target Protein: Acetylcholinesterase (ACHE) Source: Drosophila melanogaster Host: Pichia pastoris Vector: pPink α- HC Capacity: Industrial | 4 |
Target Protein: β-Glucosidase (BglI) :Source Host: Pichia pastoris Vector: pPink α- HC Capacity: Semi-industrial | 5 |
Target Protein: phytase :Source Host: Pichia pastoris X33 Vector: PpicZA Capacity: Laboratory | 6 |
Target Protein: Expression control for Pichia pastoris X33 – :Source Host: Pichia pastoris X33 Vector: PpicZA | 7 |
Target Protein: Invertase :Source Host: Pichia pastoris Vector: pPink α- HC Capacity: Semi-industrial | 8 |
Target Protein: Bovine chymosin Source: Bovine Host: Pichia pastoris X33 Vector: pPiczαA Capacity: Laboratory | 9 |
Target Protein: Vascular endothelial growth factor (VEGF) Source: Homo sapience Host: Pichia pastoris Vector: pPink α- HC Capacity: Laboratory | 10 |
Target Protein: Hydrophobin Film Structure II (HFB II) Source: Homo sapience Host: Pichia pastoris X33 (Humanized) Vector: pPiczαA HC +HFB II Capacity: Laboratory | 11 |
Target Protein: Hydrophobin Film Structure II (HFB II) Source: Homo sapience Host: Pichia pastoris X33 (Humanized) Vector: pPiczαA LC Capacity: Laboratory | 12 |
Target Protein: Alkaline Protease Source: Bacillus Clausii (native) – :Host – :Vector Capacity: Semi-industrial | 13 |
Target Protein: Alkaline Alkaline Amylase Source: Baciluus amyloliquefaciens (Native) – :Host – :Vector Capacity: Laboratory | 14 |